From: The PTS transporters of Lactobacillus gasseri ATCC 33323
Strain or plasmid | Relevant characteristics | Source or reference |
---|---|---|
L. gasseri | Â | Â |
NCK334 | ATCC 33323, human intestinal isolate | ATCC |
MJM79 | ATCC 33323 with pTRK669 | This study |
MJM75 | ATCC 33323 EI::pMJM-1, EI- | This study |
MJM99 | ATCC 33323 PTS 15::pMJM-4, PTS 15- | This study |
MJM100 | ATCC 33323 PTS 20::pMJM-5, PTS 20- | This study |
MJM101 | ATCC 33323 PTS 21::pMJM-6, PTS 21- | This study |
NCK100 | ADH, human intestinal isolate | [43] |
MJM55 | ATCC 19992 | ATCC |
E. coli | Â | Â |
EC 1000 | RepA+ MC1000, Kmr, carrying a single copy of the pWV01 repA gene in the glgB gene; host for pORI28-based plasmids | [44] |
NCK1609 | EC1000(pORI28) | [44] |
NCK1391 | EC1000(pTRK669) | [44] |
MJM80 | EC1000(pMJM-1) | This study |
MJM103 | EC1000(pMJM-4) | This study |
MJM104 | EC1000(pMJM-5) | This study |
MJM105 | EC1000(pMJM-6) | This study |
Plasmids | Â | Â |
pORI28 | Emr, ori (pWV01), replicates only with repA provided in trans | [44] |
pTRK669 | ori (pWV01), Cmr, provides repA in trans, temperature sensitive | [44] |
pMJM-1 | 2.5 kb, pORI28 with 836-bp internal L. gasseri ATCC 33323 EI fragment | This study |
pMJM-4 | 2.5 kb, pORI28 with 819-bp internal L. gasseri ATCC 33323 PTS 15 fragment | This study |
pMJM-5 | 2.4 kb, pORI28 with 760-bp internal L. gasseri ATCC 33323 PTS 20 fragment | This study |
pMJM-6 | 2.3 kb, pORI28 with 675-bp internal L. gasseri ATCC 33323 PTS 21 fragment | This study |