Table 2 Bacterial strains and plasmids used in this study.

Strains

   E. coli

DH5α

General cloning strain

Tuner(DE3)

Expression strain

Novagen

   S. mutans

ATCC 700610

UA159

Wild-type, Erm^s, Sp^s

This study

ALSM3

UA159ΔmleR, Erm^r

This study

ALSM20

UA159::ϕ(mleR_P-luc), Sp^r

This study

ALSM13

UA159ΔmleR::ϕ(mleR_P-luc), Erm^r, Sp^r

This study

ALSM33

UA159::ϕ(mleS_P-luc), Sp^r

This study

ALSM34

UA159ΔmleR::ϕ(mleS_P-luc), Erm^r, Sp^r

This study

This study

This study

Plasmids

   pFW5

Suicide vector, Sp^r

A. Podbielski [29]

   pHL222

Ap^r, luc

H. Lössner

   pALEC15

Derivate pFW5, luc, Sp^r

This study

   pALEC16

pALEC15 + ϕ(mleR_P-luc), Sp^r

This study

   pALEC47

pALEC15 + ϕ(mleS_P-luc), Sp^r

This study