Table 1 Strains and plasmids used in this study

Strains

P. stutzeri

A1501

Wild type, Chinese culture CGMCC 0351, Ben⁺⁺, Cat⁺⁺, 4HBA⁺

[22]

A1601

A1501 benR::pK18mob ΔbenR, Ben^-, Cat⁺⁺, 4HBA⁺

this study

A1602

A1501 pcaR::pK18mob ΔpcaR, Ben^-, Cat^-, 4HBA^-

this study

A1603

A1501 pcaD::pK18mob ΔpcaD, Ben^-, Cat^-, 4HBA^-

this study

A1608

A1601 harboring complement plasmid pLbenR

this study

A1609

A1602 harboring complement plasmid pLpcaR

this study

A1610

A1603 harboring complement plasmid pLpcaD

this study

Plasmids

pK18mob

Km^r; oriColE1 Mob⁺ lacZα⁺, used for directed insertional disruption

[45]

pRK2013

Km^r; Tra⁺, oriColE1

[46]

pLAFR3

Tcr; Tra-, Mob+, cos, RK2 replicon

[47]

pKbenR

Km^r; 293 bp EcoR I-Hind III fragment containing part of benR in pK18mob

this study

pKpcaR

Km^r; 299 bp EcoR I-Hind III fragment containing part of pcaR in pK18mob

this study

pKpcaD

Km^r; 361 bp EcoR I-Hind III fragment containing part of pcaD in pK18mob

this study

pLbenR

Tc^r; 1041 bp EcoR I-Hind III fragment containing benR with its native promoter in pLAFR3

this study

pLpcaR

Tc^r; 1457 bp EcoR I-Hind III fragment containing pcaR with its native promoter in pLAFR3

this study

pLpcaD

Tc^r; 820 bp EcoR I-Hind III fragment containing pcaD with the lac promoter in pLAFR3

this study