A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

Table 2 Characteristics of the fluorescent dyes used in confocal laser scanning microscopy

Fluorescent substance	Manufacturer	Excitation wavelength (Laser) in [nm]	Emission range in [nm]	Concentration/incubation time/temperature	Fluorescence of
Acridine orange	Acridine orange - zinc chloride, Applichem GmbH, Darmstadt; Germany	Argon 458	505-550 BP 592-753 BP	200 μg/mL; 2-5 min; RT	nucleic acids
DAPI	Dapi Biochemica, Applichem GmbH, Darmstadt; Germany	Diode 405	420-480 BP	20 μg/mL; 30 min; RT	nucleic acids
ConA-Alexa Fluor 488	Concanavalin A - Alexa Fluor^® 488 conjugated, Invitrogen Molecular Probes, Eugene, USA	Argon 488	505-530 BP	10 μg/mL; 30 min; RT	polysaccharides
CTC	CTC (5-Cyano-2,3-di-4-tolyl-tetraolium chloride), Polysciences Inc.; Warrington, USA	Diode 561	575 LP	1.25 mg/mL; 3 h; RT	redox activity

ISSN: 1471-2180