Table 1 Constructs and plasmids used in this study

NB10

Amp^r, pET16b, His₁₀-Ppr

[63]

pBAD18

Amp^r, araC, P_BAD, pBR322 ori, expression vector

[55]

pBAD-Pph

Amp^r, pBAD18, His₁₀-Ppr_Δ1-601-StrepTag II

this study

pBAD-PphH670A

Amp^r, pBAD18, His₁₀-Ppr_{Δ1-601; H670A}-StrepTag II

this study

pBAD-Ppr

Amp^r, pBAD18, His₁₀-Ppr-StrepTag II

this study

pBAD-KdpE

Amp^r, pBAD24, Rc-KdpE

this study

pET16b

Amp^r, T7 promoter, N-terminal deca histidyl tag

Novagen

pET16b-Pph

Amp^r, pET16b, His₁₀-Ppr_Δ1-601-StrepTag II

this study

pET16b-PphH670A

Amp^r, pET16b, His₁₀-Ppr_{Δ1-601; H670A}-StrepTag II

this study

pET16b-Ppr

Amp^r, pET16b, His₁₀-Ppr-StrepTag II

this study

pET28-CheAY

Kan^r, pET28a(+), His₆-CheAY

[12]

pRK2013

Kan^r, Tra, helper plasmid

[58]

pSK10

Gen^r, pZJD11

this study

pSK4

Amp^r, pT7-7, Ppr_Δ1-601-StrepTag

this study

pT7-7

Amp^r, T7 promoter, expression vector

[54]

pT7-7-CheW

Amp^r, pT7-7, His₆-Rc-CheW

this study

pT-Adv

Amp^r, Kan^r, lac promoter, lacZ

Clontech Inc.

pZJD11

Gen^r, pRK2 derived plasmid, lacZ

[12]