Universal and specific quantitative detection of botulinum neurotoxin genes

BMC Microbiology

Table 3 Primer and probe sets for each serotype used in quantitative PCR

Toxin Class	Sequence	Location on Toxin Gene(bp)
BoNT A Forward	TGGTTTTGAGGAGTCACTTGAA	582
BoNT A Reverse	TCATGTCCCCCAAATGTTCT	809
BoNT A Probe	TGCAGGCAAATTTGCTACAGATCCA	627
BoNT B Forward	CAAGAAAACAAAGGCGCAAG	619
BoNT B Reverse	CTGGGATCTTGYCCTCCAAA	833
BoNT B Probe	CGTGGATATTTTTCAGATCCAGCCTTG	652
BoNT C Forward	CAACTTTAATTATTCAGATCCTGTTGA	18
BoNT C Reverse	GGCTTGTAACTCGAGGAGGTT	199
BoNT C Probe	TGAGCCTGAAAAAGCCTTTCGCA	93
BoNT D Forward	CCATCATTTGAAGGGTTTGG	541
BoNT D Reverse	TGGGTCCATCTTGAGARAAA	791
BoNT D Probe	GATTCGTCCACAAGTTAGCGAGGGA	744
BoNT E Forward	ATAATGGGAGCAGAGCCTGA	448
BoNT E Reverse	CCCTTTAGCCCCATATAGTCC	678
BoNT E Probe	TGCCAAGCAATCACGGTTTTGG	515
BoNT F Forward	GTSAGACAATACCTCAAATATCAAATCG	1488
BoNT F Reverse	CTGGYACTTTTTGTGCATGT	1646
BoNT F Probe	TGCCAAGATATGATTCTAATGGAA	1551
BoNT G Forward	ATCCAACCTGGAGCTGAAGA	427
BoNT G Reverse	GCTGGATCTGCAAAATACGC	674
BoNT G Probe	TGGCCATTCCCCAATATCAGAAGG	534

Y= C or T
R= A or G
S= G or C
Indicated in this table are the type specific primers and probes for each BoNT tested in this manuscript. Included are forward, reverse and probe sequences and their locations within the toxin gene. Bases indicated in bold represent degenerate bases: Y represents C or T; S represents C or G, and R represents A or G.

ISSN: 1471-2180