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Figure 2 | BMC Microbiology

Figure 2

From: Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana

Figure 2

Expression and purification of the Tma SSB and Tne SSB. Proteins expression were obtained from the pET30Ek/LIC vector in BL(DE3)pLysS E. coli cells. Proteins were examined on 15% SDS-polyacrylamide gel. Lane M, Marker Wide Range (Sigma) with the molecular mass of proteins marked; lanes 1 and 5, soluble protein cell extracts after IPTG induction of protein expression (10 μl); lanes 2 and 6, Tma SSB and Tne SSB after heat treatment at 80°C for 20 min (10 μl); lane 3 and 7, Tma SSB and Tne SSB after chromatography on a QAE-cellulose column (10 μl); lane 4 and 8, Tma SSB and Tne SSB after chromatography on a ssDNA-cellulose column (10 μl).

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BMC Microbiology

ISSN: 1471-2180

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