PpiD is a player in the network of periplasmic chaperones in Escherichia coli

BMC Microbiology

Table 1 Plating efficiencies on SDS/EDTA

Strain	Plasmid	Efficiency of plating^a on 0.5 mM EDTA
		+ 0.5% SDS	+ 2% SDS
wild-type	None	0.90	0.54 ± 0.146
	pASK75	0.93 ± 0.061
surA	pASK75^b	8.0, 0.028, and 0.011 [× 10^-3]
	pSurA	1.0 ± 0.13
	pPpiD^b	5.8, 0.011, and 0.032 [× 10^-3]
ppiD::Tn10	None		0.66 ± 0.156
	pASK75	0.96 ± 0.087
ppiD::kan	None		0.42 ± 0.184
	pASK75	0.81 ± 0.067
surA ppiD::Tn10	pASK75^b	2.6, 7.2, and 0.66 [× 10^-3]	.
	pSurA	0.7 ± 0.02
	pPpiD^b	4.1, 2.6, and 0.25 [× 10^-3]
Δskp	None	0.87 ± 0.02	0.57 ± 0.042
	pQE60	1.04
Δskp ppiD::kan	none	1.01 ± 0.06	0.17 ± 0.042
	pQE60	1.0

^aValues are the averages of at least three independent experiments.
^bThe plating efficiencies of three independent experiments are shown, as the wide range of variation did not allow averaging. Larger variations in the efficiencies of plating were observed only for strains showing strongly increased SDS/EDTA sensitivity and likely result from minor fluctuations in the concentration of these membrane perturbants in the different batches of medium (prepared freshly for each experiment).

ISSN: 1471-2180