Table 3 Strains and plasmids

B. pseudomallei

   DD503

Parental strain; polymyxin B^R zeocin^S kanamycin^S streptomycin^R

   DD503.boaA

Isogenic boaA mutant strain of DD503; polymyxin B^R zeocin^R kanamycin^S streptomycin^R

   DD503.boaB

Isogenic boaB mutant strain of DD503; polymyxin B^R zeocin^R kanamycin^S streptomycin^R

   DD503.boaA.boaB

Isogenic boaA boaB double mutant strain of DD503; polymyxin B^R zeocin^R kanamycin^R streptomycin^S

B. mallei

   ATCC23344

Wild-type strain; polymyxin B^R zeocin^S kanamycin^S

   ATCC23344.boaA

Isogenic boaA mutant strain of ATCC23344; polymyxin B^R zeocin^R kanamycin^S

   E. coli

   EPI300

Cloning strain

   S17

Strain used for conjugational transfer of suicide plasmids from E. coli to B. pseudomallei or B. mallei

Plasmids

   pCC1™

Cloning vector; chloramphenicol resistant (Cm^R)

   pKAS46

Mobilizable suicide plasmid; kanamycin^R and ampicillin^R

   pCC1.3

pCC1-based plasmid control, does not confer adherence; Cm^R

   pSLboaA

pCC1 containing the B. mallei ATCC23344 boaA gene; Cm^R

   pSLboaAZEO

pSLboaA in which a zeocin^R marker was introduced near the middle of the boaA gene; Cm^R and zeocin^R

   pKASboaAZEO

pKAS46 containing the insert from pSLboaAZEO; zeocin^R , ampicillin^R and kanamycin^R

   pSLboaB

pCC1 containing the B. pseudomallei DD503 boaB gene; Cm^R

   pSLboaBZEO

pSLboaB in which a zeocin^R marker was introduced near the middle of the boaB gene; Cm^R and zeocin^R

   pKASboaBZEO

pKAS46 containing the insert from pSLboaBZEO; zeocin^R , ampicillin^R and kanamycin^R

   pKASboaB5'

pKAS46 containing a 0.8-kb insert which corresponds to a region located within the 5' end of the B. pseudomallei DD503 boaB ORF; ampicillin^R and kanamycin^R

   pKASboaB5'Amp^S

pKASboaB5' in which the ampicillin^R marker was removed; ampicillin^S and kanamycin^R

   pEM7ZEO

Source of the zeocin^R marker; ampicillin^R and zeocin^R