Figure 1From: Involvement of TIP60 acetyltransferase in intracellular SalmonellareplicationInteraction of SseF with TIP60. (A) Plasmids expressing the SseF devoid of putative transmembrane regions fused to the GAL4 binding domain were transformed into yeast strain AH109 expressing a fusion between the GAL4 activation domain and iTIP60164-546 (pZF1). (B) Plasmids expressing the various SseF fragments fused to the GAL4 binding domain were transformed into yeast strain AH109 expressing a fusion between the GAL4 activation domain and different TIP60 isoforms. SipA together with Plastin was used as a positive control. Yeast strains expressing the above plasmid combinations were streaked on SD-Leu-Trp (-LW) or -Leu-Trp-His+15 mM 3-AT media (-LWH). Quantitative β-galactosidase activities were measured from yeast grown in SD-Leu-Trp and expressed in Miller units.Back to article page