RNA Interference inhibits Hepatitis B Virus of different genotypes in Vitro and in Vivo

Zhang, Ya-Li; Cheng, Tong; Cai, Yi-Jun; Yuan, Quan; Liu, Che; Zhang, Tao; Xia, De-Zhen; Li, Rui-Yin; Yang, Lian-Wei; Wang, Ying-Bin; Yeo, Anthony ET; Shih, James Wai-Kuo; Zhang, Jun; Xia, Ning-shao

doi:10.1186/1471-2180-10-214

BMC Microbiology

Table 1 The characterization and screening for multiplex anti-HBV siRNA

From: RNA Interference inhibits Hepatitis B Virus of different genotypes in Vitro and in Vivo

ID	Sequence	Start Position	Off-target number^a	off-target score^a	Genome localization	Anti- Y1021	Anti- N10^b
B182	GGACCCCTGCTCGTGTTACAG	182	8	30	S, P	++	+
B183	GACCCCTGCTCGTGTTACAGG	183	3	30	S, P	-	-
B184	ACCCCTGCTCGTGTTACAGGC	184	3	30	S, P	-	-
B243	AGAGTCTAGACTCGTGGTGGA	243	3	30	S, P	+	+
B244	GAGTCTAGACTCGTGGTGGAC	244	9	30	S, P	+++	+++
B245	AGTCTAGACTCGTGGTGGACT	245	4	30	S, P	+++	+++
B246	GTCTAGACTCGTGGTGGACTT	246	4	30	S, P	-	-
B250	AGACTCGTGGTGGACTTCTCT	250	10	35	S, P	-	+
B251	GACTCGTGGTGGACTTCTCTC	251	7	35	S, P	+	++
B252	ACTCGTGGTGGACTTCTCTCA	252	2	30	S, P	++	++
B375	GGATGTGTCTGCGGCGTTTTA	375	1	25	S, P	++	++
B376	GATGTGTCTGCGGCGTTTTAT	376	7	30	S, P	+++	+++
B377	ATGTGTCTGCGGCGTTTTATC	377	5	35	S, P	+	++
B379	GTGTCTGCGGCGTTTTATCAT	379	4	35	S, P	+	+
B410	ATCCTGCTGCTATGCCTCATC	410	76	25	S, P	-	-
B415	GCTGCTATGCCTCATCTTCTT	415	54	25	S, P	+	++
B456	AAGGTATGTTGCCCGTTTGTC	456	2	30	S, P	++	++
B457	AGGTATGTTGCCCGTTTGTCC	457	1	40	S, P	-	+
B458	GGTATGTTGCCCGTTTGTCCT	458	7	35	S, P	++	++
B459	GTATGTTGCCCGTTTGTCCTC	459	15	25	S, P	++	++
B461	ATGTTGCCCGTTTGTCCTCTA	461	11	30	S, P	+	+
B1260	GCCGATCCATACTGCGGAACT	1260	2	25	EnhI, P	+	++
B1577	GTGTGCACTTCGCTTCACCTC	1577	13	30	X, P, DR1	+++	++
B1579	GTGCACTTCGCTTCACCTCTG	1579	5	25	X, P, DR1	++	++
B1581	GCACTTCGCTTCACCTCTGCA	1581	15	30	X, P, DR1	+++	+++
B1583	ACTTCGCTTCACCTCTGCACG	1583	21	30	X, P, DR1	++	++
B1787	GGAGGCTGTAGGCATAAATTG	1787	4	30	Pc, EnhII	++	++
B1788	GAGGCTGTAGGCATAAATTGG	1788	9	25	Pc, EnhII	++	+
B1789	AGGCTGTAGGCATAAATTGGT	1789	5	30	Pc, EnhII	+++	+++
B1880	AAGCCTCCAAGCTGTGCCTTG	1880	3	30	Pc	+	-
B1881	AGCCTCCAAGCTGTGCCTTGG	1881	23	25	Pc	-	-
B2389	AGAAGAAGAACTCCCTCGCCT	2389	42	25	C, P	-	+
B2390	GAAGAAGAACTCCCTCGCCTC	2390	26	25	C, P	-	+
B2391	AAGAAGAACTCCCTCGCCTCG	2391	29	25	C, P	-	-
B2392	AGAAGAACTCCCTCGCCTCGC	2392	19	30	C, P	-	+
B2393	GAAGAAGAACTCCCTCGCCTC	2393	18	30	C, P	-	+
B2394	AAGAACTCCCTCGCCTCGCAG	2394	29	25	C, P	-	+
B2395	AGAACTCCCTCGCCTCGCAGA	2395	14	35	C, P	+	+
B2396	GAACTCCCTCGCCTCGCAGAC	2396	18	35	C, P	-	+
B2397	GATCCATACTGCGGAACTCCT	2397	11	35	C, P	-	-
L1254	TGGCTACATTCTGGAGACATA	NA	NA	NA	luciferase	-	-

NA, no application.
"+" indicates weak inhibition (below 50%),
"++" indicates medium inhibition (above 50%, but below 90%),
"+++" indicates strong inhibition (above 90%),
"-" indicates no significant inhibition,
An underline represents the four candidates that were worthy for further research.
^a: off-target effects were evaluated by the online SOS program http://rnai.cs.unm.edu/offTarget.
^b: anti-HBV effects were evaluated by decreases in extracellular HBsAg level.

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