Transmission electron micrographs (TEM) of the Burkholderia bacteriophages analyzed in this project and schematic illustrations of their genomes. (A) TEM of bacteriophages negatively stained with 1% phosphotungstic acid. (B) Schematic illustrations of the P2-like Myoviridae genomes of ϕ52237, ϕE202, and ϕE12-2. Cyan shading represents sequences that are conserved in the subgroup A Myoviridae ϕ52237, ϕE202, and ϕK96243 and lime shading represents sequences that are conserved in the subgroup B Myoviridae ϕE12-2, GI15, and PI-E264-2. Gray shading represents sequences that are variably present in Myoviridae subgroups A and B. (C) Schematic illustration of the lambda-like Siphoviridae genome of ϕ644-2. Gray shading represents sequences that are unique to ϕ644-2. (D) Schematic illustration of the Mu-like Myoviridae genome of ϕE255. Gray shading represents sequences that are unique to ϕE255 and orange shading represents packaged host DNA. The 23-bp imperfect direct repeats at the left and right ends of the ϕE255 genome are shown and sequence differences with the repeat sequences of BcepMu are underlined. Genomic illustrations were obtained from the Integrated Microbial Genomes website http://img.jgi.doe.gov/cgi-bin/pub/main.cgi. Genes are shown as arrows that are pointing in their relative direction of transcription and are color coded based on their % GC composition (see scale at bottom). Individual genes with functional annotations are labeled and designated with an asterisk (*) while groups of genes with a common function are labeled and designated with a line. The locations of att sites are shown as red oblong circles. Nucleotide sequence numbering is shown above each genome.