Table 2 Bacterial strains and plasmids used in this work

Strains

E. coli

TG-1

supE hsd Δ5 thi Δ(lac-proAB) F'[traD 36]

[24]

TP611

recBC hsdR ^- M ^- cyab10pcn

[25]

B. cereus 14579

wild type isolate

[34]

B. subtilis

168

trpC2

Laboratory stock

1A717

amyE::erm Em^R

[32]

1A765

trpC2 lys

[35]

NF33

trpC2 lys amyE::pBCJ307 (P_{lysK (T box)}lacZ) Cm^R

This study

NF52

trpC2 amyE::pNF48 (P_{lysK (T box)}lysK) Spec^R

This study

NF54

trpC2 amyE::pNF48 (P_{lysK (T box)}lysK) Spec^R DlysS Kan^R

This study

NF58

trpC2 amyE::pBCJ307 (P_{lysK (T box)}lacZ) Cm^R asnS::pNF40 (P_spac asnS) Em^R

This study

NF60

trpC2 amyE::pBCJ307 (P_{lysK (T box)}lacZ) Cm^R asnS::pNF40 (P_spac asnS) Em^RpMap65 (P _pe -lacI) Ph^R

This study

NF113

trpC2 lysS::pNF112 (P_{lysK (T box)}lysS) Cm^R

This study

NF204

trpC2 amyE:: pBCJ307 (P_{lysK (T box)}lacZ) Em^R Cm^R

This study

NF205

trpC2 lysS::pNF112 (P_{lysK (T box)}lysS) Cm^R amyE:: pBCJ307(P_{lysK (T box)}lacZ) Em^R

This study

NF206

trpC2 amyE::pNF48 (P_{lysK (T box)}lysK) Spec^R DlysS: Kan^R amyE::pBCJ307(P_{lysK (T box)}lacZ)Cm^R

This study

BCJ363

trpC2 amyE::pBCJ307 (P_{lysK (T box)}lacZ) Cm^R

This study

BCJ366

trpC2 amyE::pBCJ307 (P_{lysK (T box)}lacZ) Cm^R lys::pXZ2 (P_spac lysS) Em^R

This study

BCJ367

trpC2 amyE::pBCJ307 (P_{lysK (T box)}lacZ) Cm^R lys::pXZ2 (P_spac-lysS) Em^R pMap65 Phl^R

This study

Plasmids

pBluescript2 KS(-)

cloning vector Ap^R

Stratagene, La Jolla CA

pMutin4

integration vector for B. subtilis

[27]

pBCJ102

pBluescript based vector containing transcription terminator cassettes Ap^R

[29]

pBCJ144

vector to replace part of B. subtilis lysS with Kan^R

[29]

pBCJ307

vector with transcriptional fusion of B. cereus lysK promoter and T box with lacZ Cm^R

This study

pDG268

vector to generate lacZ promoter fusions at the amyE locus by double crossover Ap^R Cm^R

[26]

pDG1730

vector for integration at the amyE locus in B. subtilis Spec^R

[30]

pXZ2

Vector to placing B. subtilis lysS under control of IPTG inducible Pspac promoter Em^R

This study

pMUTIN-XZ

pMUTIN4 with the lacZ gene removed Em^R

This study

pMap65

replicating B. subtilis plasmid encoding penP-lacI Phl^R Kan^R

[28]

pNF30

plasmid with B. cereus lysK promoter and T box element in pBCJ102 Ap^R

This study

pNF40

B. subtilis asnS promoter on 516 bp fragment in pMUTIN-XZ

This study

pNF48

B. cereus lysK promoter and lysK gene cloned into pDG1730

This study

pNF112

the lysK promoter and T box element (423 bp) fused to B. subtilis lysS (672 bp fragment)

This study