Figure 2

Streptococcus pneumoniae Choline-binding proteins. Topology of the Cbps was analyzed on R6 proteins when existing otherwise TIGR4 by SMART search of PFAM domains http://smart.embl-heidelberg.de/. Resulting general topology of the protein is figured, domains are named with PFAM nomenclature. YSIRK stands for the Gram-positive signal peptide (Pfam entry: PF04650). * refers to proteins for which the number of choline-binding repeats has been determined by crystallography, and was thus used in the table [36, 45–47]. The cloned part of the protein is included in the grey box. Protein and locus nomenclature together with the common names of the proteins, and references for their original discovery are listed in the second column. The third column figures the construct boundaries, and size of the complete protein, NC: Not Cloned. The latter columns display the positive or negative results of expression and solubility of the corresponding proteins.