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Figure 3 | BMC Microbiology

Figure 3

From: Gene encoding γ-carbonic anhydrase is cotranscribed with argC and induced in response to stationary phase and high CO2 in Azospirillum brasilense Sp7

Figure 3

Heterologous overexpression, purification and western blot analysis of recombinant Gca1 of A. brasilense (A) SDS-PAGE gel electrophoresis (15%) of uninduced (lane 2) and induced (lane 3) cell lysates of transformants harboring pSK7. The Gca1 protein overproduced in E. coli pSK7 is encircled. Low range molecular weight marker, Bangalore Genei (lane 1). (B). Purification of recombinant Gca1 of A. brasilense under denaturing conditions SDS-PAGE gel (15%) showing induced crude extract of transformant harboring pSK7 (Lane 2); Ni-NTA purified His.Tag Gca1 (Lane 3); Low range molecular weight marker, Bangalore Genei (Lane 1). (C) Western blot analysis showing cross-reactivity of purified recombinant Gca1 with antisera raised against CAM.

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BMC Microbiology

ISSN: 1471-2180

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