Table 1 Composition of the chemically defined medium (CDM) used to culture the Lactobacilli.
From: Metabolites produced by probiotic Lactobacilli rapidly increase glucose uptake by Caco-2 cells
Component | (g/L) |
|---|---|
Potassium hydrogen phosphate | 3.1 |
di-ammonium hydrogen citrate | 2.0 |
Potassium dihydrogen phosphate | 1.5 |
Ascorbic acid | 0.5 |
Potassium acetate | 10 |
Tween 80 - | 1.0 |
Heptahydrated magnesium sulphate | 0.5 |
Hydrated manganese sulphate | 0.02 |
Cobalt sulphate | 0.5 |
Calcium Nitrate | 1.0 |
Para-aminobenzoic acid | 0.002 |
Biotin | 0.01 |
Folic acid | 0.002 |
Guanine | 0.01 |
Thymine | 0.1 |
Cytidine | 0.1 |
2'-deoxyadenosine | 0.1 |
2'-deoxyuridine | 0.1 |
(ml/L) | |
Non-Essential Amino Acids Solution1 | 500 |
Essential Amino Acids Solution1 | 63.5 |
Vitamin Solution1 | 200 |