Table 2 Sequences and fluorescent dyes for primers and hybridization probes used for real-time PCR.
From: Acetic acid increases the phage-encoded enterotoxin A expression in Staphylococcus aureus
Target | Primer/probe | Nucleotide sequence (5' → 3') |
|---|---|---|
sea | ESA-1 | ACG ATC AAT TTT TAC AGC |
ToxA reverse | CCG AAG GTT CTG TAG AAG T | |
ToxA-Fluo1 | CCT TTG GAA ACG GTT AAA ACG AAT AAG AAA-FL1 | |
ToxA-Red1 | LC-R640-TGT AAC TGT TCA GGA GTT GGA TCT TCA-p2 | |
rrn | rRNA forward | TGT CGT GAG ATG TTG GG |
rRNA reverse | ACT AGC GAT TCC AGC TT | |
Probe 1 | GGA CAA TAC AAA GGG CAG CG-FL | |
Probe 2 | LC-R705-ACC GCG AGG TCA AGC A-p3 |