Table 1 Forward (FW) and reverse (RV) primers used in real-time PCR for the reference genes and for the SAP genes.
Gene | Orientation | Primer sequence (5' to 3') |
|---|---|---|
HWP1 | FW | GACCGTCTACCTGTGGGACAGT |
RV | GCTCAACTTATTGCTATCGCTTATTACA | |
ACT1 | FW | TTTCATCTTCTGTATCAGAGGAACTTATTT |
RV | ATGGGATGAATCATCAAACAAGAG | |
RPP2B | FW | TGCTTACTTATTGTTAGTTCAAGGTGGTA |
RV | CAACACCAACGGATTCCAATAAA | |
PMA1 | FW | TTGCTTATGATAATGCTCCATACGA |
RV | TACCCCACAACTTGGCAAGT | |
RIP | FW | TGTCACGGTTCCCATTATGATATTT |
RV | TGGAATTTCCAAGTTCAATGGA | |
LSC2 | FW | CGTCAACATCTTTGGTGGTATTGT |
RV | TTGGTGGCAGCAATTAAACCT | |
SAP1 | FW | AACCAATAGTGATGTCAGCAGCAT |
RV | ACAAGCCCTCCCAGTTACTTTAAA | |
SAP2 | FW | GAATTAAGAATTAGTTTGGGTTCAGTTGA |
RV | CCACAAGAACATCGACATTATCAGT | |
SAP3 | FW | CAGCTTCTGAATTTACTGCTCCATT |
RV | TCCAAAAAGAAGTTGACATTGATCA | |
SAP4 | FW | AAACGGCATTTGAATCTGGAA |
RV | CAAAAACTTAGCGTTATTGTTGACACT | |
SAP5 | FW | CCAGCATCTTCCCGCACTT |
RV | GCGTAAGAACCGTCACCATATTTAA | |
SAP6 | FW | TGGTAGCTTCGTTGGTTTGGA |
RV | GCTAACGTTTGGTCTACTAGTGCTCATA | |
SAP9 | FW | AAAGCAGCAGCGGCAGTACT |
RV | ATCCAAAACAACACCCGTGGTA | |
SAP10 | FW | CCTTATTCGAACCGATCTCCAA |
RV | CAATGCCTCTTATCAACGACAAGA |