Table 4 Oligonucleotide sequences used in this study.
Primer | Sequence (5'-3') | Gene | Comments |
|---|---|---|---|
flgE-F | GGCTAACGAGCGTGGATAAG | flgE | FP of flgE |
flgE-R | GAGCGAGCGCTAAAGTCCTA | flgE | RP of flgE |
era-F | AAGGCTAATGCGACCAGAAA | hp0517 | FP of era |
era-R | GGAGCCCTGGTGTGTCTAAA | hp0517 | RP of era |
ML022FP | CGGGATCCCGGGGCGAAAGATTGGAGATTT | hp0256 | Allelic exchange mutagenesis |
ML027RP | CCATCGTAGATCTGGGCTGC AGCGAATTTTTTCATAGAAAAATCG | hp0256 | Allelic exchange mutagenesis |
ML028FP | GCAGCCCAGATCTACGATGGGCAATTAAAAAGCGCTCTAAGAAT | hp0256 | Allelic exchange mutagenesis |
ML023RP | CGGAATTCCGTTACGCATGCAAGCCCTC | hp0256 | Allelic exchange mutagenesis |
HP0256-F2 | TATAACAAGGAGTTACAACAATGAAAAAATTCGCTTCTGTG | hp0256 | FP of hp0256 |
HP0256-R | GCGCGCATCGATTTACGCATGCAAGCCCTCTT | hp0256 | RP of hp0256 |
FLA-F2 | GCGCGCGGATCCCATGCTCCTTTAAATTTTGC | flaA | FP of flaA promoter |
FLA-R | TGTTGTAACTCCTTGTTATA | flaA | RP of flaA promoter |
minD-F | TAATTTAGCGATCGGCTTGG | minD | FP of minD |
minF-R | TCCATCACATCCACCACATC | minD | RP of minD |
hp0610-F | ATAACGGCGTTCATTCTTGG | hp0610 | FP of hp0610 |
hp0610-R | GCGGTTGTTATGCAAGGTTT | hp0610 | RP of hp0610 |
omp6-F | GCCCGATTCTAAAGGGTTTC | omp6 | FP of omp6 |
omp6-R | GGCCAAACTCTTTGGTGGTA | omp6 | RP of omp6 |
hpn-F | ATGGCACACCATGAAGAACA | hpn | FP of hpn |
hpn-R | GATGAGAGCTGTGGTGGTGA | hpn | RP of hpn |
HP0256-QF | GCGCGCCCATGG AAAAATTCGCTTCTGTATTGG | hp0256 | FP of hp0256 |
HP0256-QR | GCGCGCGGATCC TTACGCATGCAAGCCCTCTTT | hp0256 | RP of hp0256 |