Fig. 4

Induction of DAF‐16::GFP nuclear localization in C. elegans after feeding with C. neoformans and C. gattii. A Representative image of DAF-16 nuclear localization of E. coli OP50-, C. neoformans-, and C. gattii-infected daf-16p::GFP (zIs356) nematodes. All worms were exposed to E. coli OP50, C. neoformans 32045, and C. gattii 56992 on BHI plates for 48 h at 25 °C, then imaged. B Relative DAF-16::GFP nuclear accumulation was evaluated by using ImageJ software, and the mean DAF-16::GFP nuclear accumulation of mock-infected nematodes was set as 1. Data were represented in mean ± SD (n = 30). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; not significant (P > 0.05). Scale bar = 100 µm