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Fig. 6 | BMC Microbiology

Fig. 6

From: Quantitative flow chamber system for evaluating in vitro biofilms and the kinetics of S. aureus biofilm formation in human plasma media

Fig. 6

Kinetics of 16S rRNA for UAMS-1 and USA300 in the in vitro biofilm formation on stainless steel. Bioluminescent UAMS-1 and USA300 (USA300 LAC:lux) were inoculated in the flow chamber as described in Figs. 3 and 4, and the stainless-steel pins were harvested at the indicated time (n = 4). RNA was extracted and after that cDNA was generated by iScript kits. Electrophoresis and quantitative real-time PCR were done using 16S rRNA primers. A Images from the agarose gel electrophoresis are shown for UAMS-1 (left-side), and USA300 (right side). B Graph of the quantification real-time PCR analyses of UAMS-1 and USA300. Note that a significant amount of 16S rRNA occurred at 3 h and then increased in a time-dependent manner for both strains. UAMS-1 had its peak at 9 h, while USA300 peaked at 6 h (*p < 0.05 vs. other inoculation times in UAMS-1; #p < 0.001 vs. other inoculation times in USA300)

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