Table 2 Primers used in this work
Primer | Sequence (5′ - > 3′) |
|---|---|
Mutant and overexpressing strains | |
CV3905-del1 | TAGGGCCCGAAACTGCGGCTCGAACGCC |
CV3905-del2 | ATCAAGCTTCATCGAAAGAGTCCCCGTGC |
CV3905-del3 | ATCAAGCTTGAAAACACGCCGGCCTGAGC |
CV3905-del4 | TTGGATCCCTGGTGGCGGGCTTTGTTGC |
CV_3905-Comp-Fw | GGTACCCTGCAGAGCCAGTCCGCATTAAGGCG |
CV_3905-Sup-Rv | GGTTACGAGCTCGACGCTCAGCGTCAGAGCTG |
Northern blot | |
CV0209-NB-Fw | TGCAGGTGAAGTTGAGCACCCC |
CV0209-NB-Rv | CCGATGAAGCAGGCGGAATAGCC |
NB CV2534 -Fw | GATGGTGCTGATCGCGATGG |
NB CV2534 -Rv | CCAGGGTCTCGATCAGCATC |
NB CV2541-Fw | CCATTCTGTCCGCGCTGTTG |
NB CV2541-Rv | GCATTTCGTCCATGTCGCGG |
NB CV3659-Fw | CACCTCTGGTGCAGGCTTTC |
NB CV3659-Rv | GACAGCGACTGGGTGAACTG |
NB CV0568-Fw | ACGCCAAGCTGGGACTCAAC |
NB CV0568-Rv | GCTCTACCTCCCGTTCGATG |
EMSA | |
pCV0209-Fw | TTGGATCCTGCCGGAGCGGAATGCAACT |
pCV0209-Rv | ATTACTGCAGGCGGTGGCTTCGGCGGTATAC |
pCV3905-Fw | GGTACCCTGCAGAGCCAGTCCGCATTAAGGCG |
pCV3905-Rv | ATCAAGCTTCATCGAAAGAGTCCCCGT |
pCV0210-Fw | CTCGCTGGCCGATCTGGGAC |
pCV0210-Rv | GGCGGAATACAGCGCGAAGC |
pCV1486Fw | GACCAAGTGATAGACGCCGG |
CV1486-DEL2 | GGCCTAAAGCTTCTCGGTCATGAGGATGTCTG |
pCV2087Fw | CCTCGTAAAAGCGGATGGCG |
pCV2087Rv | CGTCGAAACCCTTCATCGCG |
pCV2534Fw | GATCACCGACACCCTCAAGC |
pCV2534Rv | GCGACAACACCAGCAGCTTG |
pCV2545Fw | TGACTCTCCCCCATTGGGAG |
pCV2545Rv | ATCGGGATGCCCAGCACCGC |
pCV3659Fw | GACCGACAATGACGGCTACG |
pCV3659Rv | GCCGAAATGCAGCACGAAAG |
CV0208 Fw | GACCATACTAGACAGCTACGCC |
CV0208Rv | CAGCGTGGTGATTTGCGGATAG |