Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon

Table 3 Phylogenetic relationships between S. aureus clonal complexes, using the concatenated gene sequences from the MLST database

Microrganism	Gene	Nucleotide composition (%)				Lenght (n)	Variable sites (S)	S/n (Ps)	Θ	π	Tajima’s D
Microrganism	Gene	A	C	G	T	Lenght (n)	Variable sites (S)	S/n (Ps)	Θ	π	Tajima’s D
S. aureus	arcC	36.4	17.1	21.4	25.2	456	67	0.1469	0,0186	0.0068	−1.6654
	aroE	36.0	11.3	18.9	33.8	456	70	0.1535	0.0205	0.0099	−1.3612
	glpF	25.7	16.3	24.5	33.5	465	112	0.24	0.0318	0.0044	−2.3522
	gmK	37.2	12.4	21.2	29.3	417	53	0.127	0.0164	0.0083	−1.2761
	ptA	35.8	16.6	19.5	28.0	474	196	0.4135	0.0618	0.0068	−2.4876
	tpI	36.6	15.1	22.6	25.8	402	139	0.3458	0.0487	0.0096	−2.2167
	yqiL	34.0	15.6	22.4	28.0	516	68	0.1318	0.0162	0.0065	−1.5817
S. epidermidis	arcC	36.8	19.2	17.0	27.1	468	72	0.1538	0,0190	0.0153	−0.5227
	aroE	37.1	11.8	17.8	33.3	420	78	0.1857	0.0230	0.0110	−1.3911
	gtr	28.7	20.4	14.9	36.0	438	86	0.1963	0.0244	0.0119	−1.3708
	mutS	30.4	19.8	11.4	38.4	412	48	0.1165	0.0144	0.0054	−1.6011
	pyrR	34.4	16.1	21.9	27.5	424	57	0.1344	0.0167	0.0086	−1.2572
	tpiA	34.6	16.0	22.0	27.4	424	67	0.1580	0.0196	0.0036	−2.1526
	yqiL	31.6	15.2	21.5	31.6	416	62	0.1490	0.0185	0.0079	−1.4948

Nucleotide frequencies and parameters associated with the Tajima neutrality test for each MLST gene analysed. A, C, G and T are the percentages for each nucleotide in the genes in question. Size (n) corresponds to the total number of sites in each gene. Variable sites (S) correspond to the sites that showed nucleotide changes during alignments. Ps corresponds to the ratio S/n. Θ and π correspond to values that measure nucleotide diversity. Tajima’s D corresponds to the reference parameter to verify the neutral theory of evolution for each gene. All values of Tajima’s D showed statistical significance P < 0.01

ISSN: 1471-2180