Fig. 2From: Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancerQuantitative polymerase chain reaction (qPCR) analysis of bacterial markers in formalin-fixed paraffin embedded (FFPE) tissues. qPCR results of specific bacterial markers in colorectal cancer (CRC; n = 14), normal adjacent (ADJ; n = 12) and healthy colonic control (C; n = 13) using non-normalized template. The relative amount of each sample is normalized to human genomic DNA and expressed in terms of fold change (2−ΔΔCT). a-b Detection of Escherichia coli (E.coli) (a) and strains harbouring the pks + island (b) using E. coli and ClbA gene specific primers, respectively. c Detection of Faecalibacterium prausnitziiBack to article page