Table 1 Primer sequence and qPCR conditions used for relative quantification analysis
|  | Primer sequence 5′-3′ | qPCR program |
|---|---|---|
Streptococcus mutans | Forward: CTACACTTTCGGGTGGCTTG | 95 °C 2 min, |
Choi et al., 2009 [18] | Reverse: GAAGCTTTTCACCATTAGAAGCTG | 40 × 95 °C 10s, 61 °C 20s, Plate read |
Total bacteria (reference gene) | Forward: TGGAGCATGTGGTTTAATTCGA | 94 °C 4 min, |
Choi et al., 2009 [18] | Reverse: TGCGGGACTTAACCCAACA | 40 × 94 °C 20s, 62 °C 20s, Plate read |
Total lactobacillus | Forward: TGGAAACAGRTGCTAATACCG | 98 °C 2 min, |
Roy et al., 2004 [19] | Reverse: GTCCATTGTGGAAGATTCCC | 40 × 98 °C 10s, 62 °C 15 s, Plate read |
Lactobacillus reuteri DSM 17938 | Forward: TTAAGGATGCAAACCCGAAC | 98 °C 2 min, |
Vestman et al., 2013 [20] | reverse: CCTTGTCACCTGGAACCACT | 40 × 98 °C 5 s and 60 °C 15 s, Plate read |
Lactobacillus reuteri PTA 5289 | Forward: GACAGTGGCTAAACGCCTTC | 98 °C 2 min, |
Vestman et al., 2013 [20] | Reverse: AATTCCACTTGCCATCTTCG | 40 × 98 °C 5 s and 60 °C 15 s, Plate read |
Total Streptococci | Forward: YGTGCAATTTTTGGATAAT | 95 °C 3 min, |
Täpp et al., 2003 [21] | Reverse: TTCTATAAGCCATGTTTTGT | 40 × 94 °C 20s, 52 °C 30s, Plate Read |