Fig. 5
Effect of silencing of gC1qR gene on TcdA-induced mitochondrial dysfunction in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Intracellular ROS generation was measured by fluorescence of H2DCFDA (Green). b NCM 460 cells were stained with JC-1 and were subjected to flow cytometry. The relative Δψm value was examined by monitoring the fluorescence of JC-1 (590: 527 nm fluorescence ratio). c The ATP levels in NCM 460 epithelial cell were assessed. The data are presented as mean ± S.D. (n = 3). **p < 0.01, #p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group; △△p < 0.01versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group