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Table 2 List of primers used in this study and for detection of Mycobacterium smegmatis (NCIMB 8548), M. smegmatis environmental isolate (VS/02) and genomic DNA from Mycobacterium tuberculosis (ATCC®25177).

From: A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides

Primer

Primer sequences (5′-3′)

Gene

Location

Annealing temperature (°C)

Sequence reference (GenBank accession number)

Product sizes (bp)

References

BMS 738aF

GAC AAG TCC ACC GAG AAG AC

rpoB

738–758

59

AY262735 U24494

593

Present study

BMS 1311aR

ATC TGG TTC TGG ATC AGC TC

rpoB

1311–1331

Present study

BnMS 949bf

TGG AGA AGG ACA CCA CCT

rpoB

949–967

59

AY262735 U24494

179

Present study

BnMS 1105br

CAG CTT CTT GTT GAC CTT GT

rpoB

1105–1125

Present study