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Fig. 2 | BMC Microbiology

Fig. 2

From: Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains

Fig. 2

Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): (a) Staphylococcus aureus ATCC 29213, (b) Staphylococcus aureus MRSA ATCC 43300, (c) Enterococcus faecium ATCC 35667, (d) Enterococcus faecium VRE ATCC 700221, (e) Enterococcus faecalis ATCC 29212, (F) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min

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