Fig. 3From: Development of flow cytometric opsonophagocytosis and antibody-mediated complement deposition assays for non-typeable Haemophilus influenzaeSelection of incubation time for CDA. NTHi strain 3655 was incubated with 2% IgG-depleted human plasma along with human pre and post Synflorix sera. Antibody-dependent (a) C3b/iC3b and (b) C5b-9 binding was determined by flow cytometry and expressed as MFI-control following incubation for 20 or 45Â min. Significance was determined by two tailed t-testBack to article page