Table 2 Estimated mean MAP load determined by qPCR at different dilutions (10−2, 10−4 and 10−6) before (PRE) and after (POST) treatment with copper ions, for each replicate experiment. Data represent mean number of MAP DNA copies (genome equivalents) per 200 μL of PBS, from which DNA was extracted
From: In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions
Replicate experiment | MAP dilution: | |||||
|---|---|---|---|---|---|---|
10−2 | 10−4 | 10−6 | ||||
PRE | POST | PRE | POST | PRE | POST | |
I | 1.9 × 106 | 1.4 × 103 | 1.5 × 103 | 3.2 × 102 | 1.3 × 102 | NDa |
II | 2.8 × 106 | ND | 5.5 × 102 | ND | 4.7 × 101 | ND |
III | 9.4 × 105 | 1.2 × 101 | 9.2 × 102 | 1.6 × 101 | 3.3 × 102 | ND |
IV | 1.9 × 105 | 1.1 × 101 | 1.5 × 103 | 1.2 × 101 | 2.7 × 102 | ND |
V | 1.6 × 105 | 3.2 × 102 | 3.3 × 103 | 2.1 × 101 | 3.1 × 103 | ND |