Table 1 Summary of phage assay counts (PFU mL−1) for three dilutions of MAP-spiked PBS before (PRE) and after (POST) treatment with copper ions at 24 V for 5 min; indicating significant reductions (P = 0.03) in numbers of viable MAP
From: In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions
Replicate experiment | MAP PBS dilutiona: | |||||
|---|---|---|---|---|---|---|
10−2 | 10−4 | 10−6 | ||||
PRE | POST | PRE | POST | PRE | POST | |
I | 5.8 × 108 | 3.0 × 107 | 4.5 × 106 | < 1 | 9.1 × 104 | < 1 |
II | 4.6 × 108 | 2.0 × 107 | 3.0 × 106 | < 1 | 6.3 × 104 | 4.0 × 103 |
III | 5.6 × 108 | < 1 | 3.3 × 106 | < 1 | 5.3 × 104 | < 1 |
IV | 3.5 × 108 | < 1 | 3.1 × 106 | < 1 | 5.0 × 104 | < 1 |
V | 3.0 × 108 | < 1 | 3.0 × 106 | < 1 | 3.0 × 104 | < 1 |
VI | 7.1 × 108 | 2.0 × 107 | 3.0 × 106 | < 1 | 3.0 × 104 | < 1 |
Mean PFU mL−1 ± SD | 4.9 × 108 ± 1.5 × 108 | 1.2 × 107 ± 1.3 × 107 | 3.3 × 106 ± 5.9 × 105 | < 1 | 5.3 × 104 ± 2.3 × 104 | 6.7 × 102 ± 16 × 102 |