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Fig. 3 | BMC Microbiology

Fig. 3

From: Novel human monoclonal antibodies targeting the F subunit of leukocidins reduce disease progression and mortality caused by Staphylococcus aureus

Fig. 3

YG8–1, YG8–2, and YG8–3 bound to all F components. (a) YG8–1, YG8–2, and YG8–3 bound to HlgB, LukF, and LukD, but not HlgA. HlgA, HlgB, LukF, and LukD were coated (1 μg/well) onto 96-well plates. Bound IgG was detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG. Data are presented as the mean ± SD of three independent experiments. (b, c) The binding affinities of YG8–1, YG8–2, and YG8–3 to LukF (b) or LukD (c) were determined by ELISA. LukF or LukD was coated onto 96-well plates in the presence of serial dilutions of YG8–1, YG8–2, and YG8–3. Bound IgG was detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG

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BMC Microbiology

ISSN: 1471-2180

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