Fig. 2From: Novel human monoclonal antibodies targeting the F subunit of leukocidins reduce disease progression and mortality caused by Staphylococcus aureusYG8–1, YG8–2, and YG8–3 bound to the same region of HlgB. (a) The binding affinities of YG8–1, YG8–2, and YG8–3 to HlgB were determined by ELISA. HlgB was coated onto 96-well plates in the presence of serial dilutions of YG8–1, YG8–2, and YG8–3. Bound IgG was detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG. (b–d) YG8–1, YG8–2, and YG8–3 competed with each other in a concentration-dependent manner. YG8–1 was coated onto 96-well plates, and YG8–2 (b) or YG8–3 (c) was added at various concentrations after pre-incubated with GST-HlgB. YG8–2 was coated onto 96-well plates, and YG8–3 (d) was added at various concentrations after pre-incubation with GST-HlgB. Bound GST-HlgB was detected using an anti-GST antibody. Data are representative of three independent experiments and shown as the mean ± SDBack to article page