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Table 3 Analysis of ITS1 sequence length, microsatellite markers and Wolbachia status in Glossina laboratory populations

From: Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species

Glossina species

Country of origin (Location)

No

ITS1 expected size

Wolbachia

Microsatellites

Correctly identified samples

cytoplasmic

chromosomal

A10

Gmm14

G. pallidipes

IPCL

Uganda

8

920

0.0 % (0/8)

0.0 % (0/8)

-

+

8/8

IPCL

Ethiopia

8

12.5 % (1/8)

0.0 % (0/8)

-

+

8/8

G. m. morsitans

IPCL

8

775

75 % (6/8)

100 % (8/8)

-

+

8/8

G. m. centralis

IPCL

8

~800 + ~150

100 % (8/8)

0.0 % (0/8)

-

+

8/8

G. m. submorsitans

CIRDES

12

~800 + ~150

0.0 % (0/12)

0.0 % (0/8)

-

+

12/12

G. p. gambiensis

IPCL

POUT

8

543

0.0 % (0/8)

0.0 % (0/8)

+

+

8/8

IPCL

CIRDES

8

0.0 % (0/8)

0.0 % (0/8)

+

+

8/8

G. f. fuscipes

IPCL

Central Africa Republic

8

618

12.5 % (1/8)

0.0 % (0/8)

Partial

+

8/8

G. brevipalpis

IPCL

8

778

75 % (6/8)

0.0 % (0/8)

-

-

8/8

G. tachinoides

CIRDES

12

597

0.0 % (0/12)

0.0 % (0/8)

-

+

12/12

  1. -: no amplicon detected
  2. +: the expected amplicon was detected in all individuals screened
  3. Partial: the expected amplicon was detected, but not in all individuals screened