High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.)

BMC Microbiology

Table 1 List of Glossina and Drosophila strains analyzed in this study

	Species	Species group	Strain/abbr.	Origin/reference
Drosophila strains	Drosophila simulans	simulans	NouméaTC	[18]	Wolbachia negative control
Drosophila strains	Drosophila willistoni	willistoni	P98	[19]	Wolbachia positive control
					Wolbachia status (experimental)
					wsp	ARM	blot	FISH
Glossina strains	Glossina morsitans morsitans	morsitans	Gmm	Takáč Lab, Slovak Academy of Sciences, Bratislava, Slovakia	+++	+++	+++	+++
	Glossina morsitans centralis	morsitans	Gmc	Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria	+++	+++	+++	+++
	Glossina swynnertoni	morsitans	Gsw	Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria	–	++	++	nd
	Glossina palpalis palpalis	palpalis	Gpp	Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria	–	–	–	–
	Glossina palpalis gambiensis	palpalis	Gpg	Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria	–	–	+	+
	Glossina fuscipes fuscipes	palpalis	Gfu	Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria	–	–	+?	nd

Two Drosophila strains were used as Wolbachia-positive and -negative controls (P98, NouméaTC). The table lists all Glossina strains used for experiments, including Wolbachia infection status based on PCR (Wolbachia outer surface protein gene, Wolbachia A-supergroup repeat motif,); blot-PCR using a wsp probe (listed as ‘blot’), and fluorescence in situ hybridization with Wolbachia 16-23S rRNA probe. Wolbachia infection titer is indicated by ‘+’ (low), ‘++’ (intermediate), and ‘+++’ (high). Abbreviations: wsp Wolbachia outer surface protein gene, ARM Wolbachia A-supergroup repeat motif, FISH fluorescence in situ hybridization, nd not determined

ISSN: 1471-2180