Fig. 3
From: High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.)
Stellaris® rRNA-FISH on high-titer Glossina species (G. m. morsitans, G. m. centralis). a In the ovary Wolbachia are present in the first and in the second ovariole plus at the posterior pole of the oocyte. b Wolbachia are not detected in early stages of spermatogenesis (hub cells, spermatogonia) but are visible in later stages (spermatocytes, spermatids). Also the spermatheca, particularly the sheath cells (c, f), as well as adipocytes (d) and milk gland (e) are infected with Wolbachia. (e’) Wigglesworthia, primary symbiont of Glossina, are also present in the milk gland. Wolbachia are stained in pink (16-23S rRNA), Wigglesworthia are shown in green (16-23S rRNA), Glossina DNA is stained in blue (4′,6-diamidino-2-phenylindole), brightfield images for better orientation are presented for a-f. Scale bar is 100 μm