Fig. 1
From: High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.)
Detection of Wolbachia in Glossina females and males via polymerase chain reaction (PCR). a Wolbachia-specific single copy wsp-PCR detects the symbiont only in high-titer Gmm (♀, ♂) and Gmc (♀, ♂). b The more sensitive multicopy ARM-PCR additionally detects wGsw (Wolbachia of G. swynnertoni). In the Wolbachia-uninfected G. f. fuscipes, ARM-PCR amplifies a possibly nonspecific product of smaller size. Negative controls are Wolbachia-uninfected Drosophila simulans, NouméaTC (Dsimminus) and non-template control (NTC). Females are first on gel. Each PCR was at least repeated once in order to confirm results. Abbreviations: wsp Wolbachia outer surface protein gene