Fig. 2From: Analysis of the gut-specific microbiome from field-captured tsetse flies, and its potential relevance to host trypanosome vector competenceMeasurement of bacterial α and β-diversity in midguts of G. pallidipes captured in Shimba Hills, Nguruman and Murchison Falls. Plots (a) and (b) present bacterial α-diversity (indicative of species richness), which was measured using the ‘observed species metric’. a Rarefaction curves demonstrate the analysis achieved adequate sequencing depth and OTU discovery. b At a 95% confidence level (α = 0.05), a statistically significant difference in species richness was observed between flies collected from Shimba Hills vs. Murchison Falls (p = 0.024). Plot (b) shows bacterial β-diversity measured using the unweighted UniFrac metric and Bray Curtis method. c Average UniFrac distance within each collection site (left graph) and between each collection site (right graph). β-diversity was statistically significantly different between G. pallidipes captured in Shimba Hills and Murchison Falls (nonparametric paired t-test; p = 0.028). β-diversity between the other two sites was not significantly different at a 95% confidence levelBack to article page