Fig. 5
Evaluating transmission to the offspring after introduction in larval stages. recSodalis was found to be present in different tissues of 1st, 2nd and 3rd generation of male (M) and female (F) offspring resulting from female flies microinjected with 1 × 106 CFU recSodalis at the larval stage. Graphs depict the number of recSodalis CFU (DNA equivalent) present in female and males tissues (green
) versus the total number of Sodalis CFU (DNA equivalent) (WT + recombinant Sodalis) (blue
). Data points represent the mean recSodalis and total Sodalis CFU (± SD) present in the different tissues of individual flies (n = 5) at the time of sampling; 4 days post-eclosion. The number of CFU is represented in log scale on the y-axis