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Table 4 The combination effect of the treatments of PMA with triton x-100 or sarkosyl on live S. aureus cells assessed by qPCR

From: An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR

PMA concentration (μM)

Triton x-100 (0.5%)

Sarkosyl (0.025%)

1

27.33 ± 0.12a

28.64 ± 0.13

4

27.94 ± 0.04 b

29.10 ± 0.04

10

27.71 ± 0.25

29.65 ± 0.34

40

27.94 ± 0.04

29.54 ± 0.40

100

28.76 ± 0.13

28.64 ± 0.13

NCc

23.15 ± 0.10

26.24 ± 0.09

  1. arefers to the mean Cqvalue of the triplicates ± standard deviation
  2. bbold-faced number refers to a value of a sample treated with the same concentration of PMA that demonstrated a significant difference (p < 0.05) between the two surfactants used
  3. cNC refers to a negative control that was treated only with a surfactant but no PMA treatment
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BMC Microbiology

ISSN: 1471-2180

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