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Table 3 Influence of the treatment of sarkosyl or triton × − 100 on the removal efficiency of the DNA of dead cells in the PMA-qPCR assay

From: An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR

PMA concentration (μM) Sarkosyl (0.025%) Triton × − 100 (0.5%) PBS
4 31.68 ± 0.35 a b 32.53 ± 0.20 29.94 ± 0.33
10 33.40 ± 0.49 33.13 ± 0.56 31.58 ± 0.19
40 34.38 ± 0.46 33.64 ± 0.43 32.31 ± 0.31
100 34.88 ± 0.46 33.88 ± 0.34 33.20 ± 0.13
NCc 30.52 ± 0.52 29.79 ± 0.42 28.65 ± 0.35
  1. arefers to the mean Cq value of triplicate ± standard deviation
  2. bbold-faced number refers to a value of a sample treated with the same concentration of PMA that demonstrated a significant difference (p < 0.05) between the two surfactants used
  3. crefers to negative controls, which were treated only with surfactant or PBS but without PMA