An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR

Table 3 Influence of the treatment of sarkosyl or triton × − 100 on the removal efficiency of the DNA of dead cells in the PMA-qPCR assay

PMA concentration (μM)	Sarkosyl (0.025%)	Triton × − 100 (0.5%)	PBS
4	31.68 ± 0.35 ^a ^b	32.53 ± 0.20	29.94 ± 0.33
10	33.40 ± 0.49	33.13 ± 0.56	31.58 ± 0.19
40	34.38 ± 0.46	33.64 ± 0.43	32.31 ± 0.31
100	34.88 ± 0.46	33.88 ± 0.34	33.20 ± 0.13
NC^c	30.52 ± 0.52	29.79 ± 0.42	28.65 ± 0.35

^arefers to the mean Cq value of triplicate ± standard deviation
^bbold-faced number refers to a value of a sample treated with the same concentration of PMA that demonstrated a significant difference (p < 0.05) between the two surfactants used
^crefers to negative controls, which were treated only with surfactant or PBS but without PMA

ISSN: 1471-2180