Fig. 9

DNA binding by V.NgoAXIII, V.NgoAXIV endonucleases and MutS protein. DNA binding by (a) V.NgoAXIII endonuclease in the absence of 1 mM ATP; b V.NgoAXIV endonucleases in the absence of 1 mM ATP; c MutS protein in the absence of 1 mM ATP; d MutS protein in the presence of 1 mM ATP. The presented results were obtained using M.NgoA302P-sub (GTCGGT/ACCGGC) for V.NgoAXIII and M.NgoA1175P-sub (CTGG/CCGG) substrate DNA for V.NgoAXIV. Lanes are indicated as follows: M - GeneRuler™ DNA Ladder Mix (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1200, 1500, 2000, 2500, 3000, 3500, 4000, 5000, 6000, 8000 and 10,000 bp) (Thermo Scientific), 1–0 μM protein, 2–0.15 μM protein, 3–0.3 μM protein, 4–0.45 μM protein, 5–0.6 μM protein, 6–0.75 μM protein. The 94-bp competitor DNA was used in the experiments, which were performed in triplicate. Representative images are shown