Fig. 3
Interactions between the gonococcal Vsr endonucleases, MutL and MutS proteins studied by protein affinity chromatography. Analysis of the interactions between (a) V.NgoAXIII and MutLNgo; b V.NgoAXIII and MutS; c V.NgoAXIV and MutLNgo; d V.NgoAXIV and MutS; and (e) MutS and MutLNgo. V.NgoAXIII, V.NgoAXIV or MutLNgo proteins were coupled to affinity gel as ligands, and subsequently His-tagged MutS or MutLNgo were applied to the resin. Representative Western blots with antibodies against His-Tag are presented. The arrows and asterisks indicate protein only in the elution fraction. P – purified proteins used in the experiment; F – flow through fraction; W - wash fraction; E - elution fraction; M - PageRuler™ Prestained Protein Ladder (250, 130, 95, 72, 55, 43, 34, 26, 15 and 11 kDa) (Thermo Scientific)