Fig. 4

Total proteome analysis of cell-free extracts from the cells of D. anilini grown on phenol (black bars) and benzoate (grey bars). (A) label-free quantification intensity (LFQ-intensity) of the enzymes encoded by pps-ppc gene cluster (locus tag 02049–02059) and putative ppsγ (locus tag 03126) and ppcα (locus tag 03563) in D. anilini grown with phenol or benzoate. (B) LFQ-intensity of enzymes (6-hydroxycyclohex-1-ene-1-carboxyl-CoA dehydrogenase, 6-ketocyclohex-1-ene-1-carbonyl-CoA hydrolase, cyclohexa-1, 5-diene-1-carbonyl-CoA hydratase, tungsten-dependent benzoyl-CoA reductase subunit bamB) involved in benzoyl CoA metabolism in D. anilini grown with phenol or benzoate. The LFQ intensity value of the enzymes from phenol-grown cells are labeled at the end of the columns. The lowest LFQ intensity value observed in total proteomics analysis was 109,120, and the highest LFQ intensity observed is 16,493,000,000