Skip to main content
Fig. 3 | BMC Microbiology

Fig. 3

From: The therapeutic potential of bacteriophages targeting gram-negative bacteria using Galleria mellonella infection model

Fig. 3

Impact of multiple phage doses on survivability of larvae that was infected with E. cloacae el140 and the efficiency of phage cocktail in treating multiple bacterial infections. The multiple doses of phages (104 PFU/mL) were injected at 0th, 6th, 12th, 24th and 30th hour after the larvae was pre-infected with 108 CFU/mL of bacteria. All the phage treatment results were compared with the control. a Impact of multiple doses of Enterobacter phage ELP140 on the survivability of larvae infected with E. cloacae el140 (p = 0.0003). a1 Bacterial enumeration from larvae, results are represented as CFU of bacteria/larva. b Phage cocktail studies- Impact of multiple doses of phage cocktail on the survivability of larvae infected with mixed bacteria (E. coli ec311, K. pneumoniae kp235 and E. cloacae el140), for the phage cocktail studies five doses of prepared phage cocktail was used and the results are represented as time to death (p = 0.0016). b1) Phage cocktail studies- Bacterial enumeration from larvae after the experiment. Results are represented as CFU of bacteria/larva. The survival rates were plotted using the Kaplan-Meier method and log-rank test was used to analyze the difference in survival rates. A statistically significant difference (p < 0.05) was observed using 10 worms per group on phage treatment. Error bars represent standard error of the mean (SEM) of three independent replicates and data were analyzed using GraphPad Prism 7.0

Back to article page