Fig. 2
Impact of multiple phage doses on survivability of larvae that was infected with E. coli ec311 and K. pneumoniae kp235. The multiple phage doses (104 PFU/mL) were injected at 0th, 6th, 12th and 24th hour after the larvae were pre-infected with 108 CFU/mL of bacteria. All the phage treatment results were compared with the control. a Impact of multiple doses of Escherichia phage ECP311 on the survivability of larvae infected with E. coli ec311 (p = 0.0002). a1 Bacterial enumeration from larvae, results are represented as CFU of bacteria/larva. b Impact of multiple doses of Klebsiella phage KPP235 on the survivability of larvae infected with K. pneumoniae kp235 (p = 0.0001). b1 Bacterial enumerations from larvae, results are represented as CFU of bacteria/larva. The survival rates were plotted using the Kaplan-Meier method and log-rank test was used to analyze the difference in survival rates. A statistically significant difference (p < 0.05) was observed using 10 worms per group on phage treatment. Error bars represent standard error of the mean (SEM) of three independent replicates and data were analyzed using GraphPad Prism 7.0