Modelling the metabolism of protein secretion through the Tat route in Streptomyces lividans

BMC Microbiology

Table 3 summary of changes observed in the Tat-secreting strain using model iJV1220

Subsystem	Tat-secreting vs. Sec-secreting				Tat-secreting vs. Plasmid-bearing
Subsystem	N	N_AMN	P _{alt = Tat < Sec}	P _{alt = Tat > Sec}	N	N_AMN	P_{alt = Tat < pIJ486}	P_{alt = Tat > pIJ486}
Amino acid metabolism	187	81	0.228	0.773	187	82	0.044	0.956
Carbon metabolism	131	17	0.333	0.679	131	18	0.419	0.594
Cell envelope	577	292	0.000	1.000	577	292	0.000	1.000
TCA	17	11	0.179	0.838	17	10	0.153	0.864
Cofactor biosynthesis	214	148	0.000	1.000	214	148	0.000	1.000
Exchange	216	43	0.646	0.357	216	45	0.578	0.425
Glycolysis and gluconeogenesis	21	13	0.185	0.829	21	12	0.397	0.625
Inorganic Ion Transport and Metabolism	59	15	0.270	0.744	59	15	0.230	0.782
Nucleotide metabolism	125	47	0.023	0.978	125	47	0.023	0.978
Oxidative Phosphorylation	17	7	0.185	0.847	17	6	0.188	0.852
PPP	15	10	0.741	0.285	15	8	0.604	0.437
Transport, Membrane	167	22	0.500	0.509	167	25	0.431	0.577
Unassigned	16	4	0.500	0.614	16	4	0.500	0.614
Amylase secretion	5	4	0.009	0.996	5	0	NA	NA
Agarase secretion	5	4	0.996	0.009	5	4	0.996	0.009

Comparisons of the Tat-secreting strain using the model iJV1220 based on MTF analysis of the AMN with pathways grouped in broad subsystems. N is the number of reactions in the subsystem, N_AMN is the number of subsystem reactions that are active in either of the two strains being compared, P is the P value obtained using the specified alternative hypothesis (i.e. when P < 0.05 the specified alternative hypothesis cannot be rejected). Only subsystems with N_AMN > 4 are reported
Abbreviations: TCA cycle tricaboxylic acid (Krebs) cycle, PPP pentose phosphate pathway, NA not applicable

ISSN: 1471-2180