Transmating: conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol

Table 1 Yield of colonies obtained in representative transmating experiments with each acceptor strain

Species	number of colonies with typical morphology
B. subtilis	> 5000 *
P. polymyxa	1750
B. vallismortis	849
B. pumilus	655
B. mojavensis	574
B. sonorensis	178
B. licheniformis	37
B. mycoides	n. q. **
B. pseudomycoides	n. q. **
B. oleronius	0
B. megaterium	0

Only colonies with the typical morphology of the respective acceptor strain were counted. On selection plates with polymyxin B concentrations ≤10 μg/ml, additional growth of E. coli was observed. *: for B. subtilis, several thousand distinct colonies and partially confluent growth were observed. **: for B. mycoides and B. pseudomycoides, the exact number of colonies could not be quantified (n.q.), due to confluent growth of large, mycelium-like colonies (diameter > 10 mm)

ISSN: 1471-2180