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Fig. 2 | BMC Microbiology

Fig. 2

From: Functions of CaPhm7 in the regulation of ion homeostasis, drug tolerance, filamentation and virulence in Candida albicans

Fig. 2

Subcellular localization and mouse virulence assay for CaPhm7. a Functional assay of the CaPHM7-GFP allele. The wild type CAI4 (XDCA06), the heterozygous mutant PHBCA100, the homozygous mutant PHBCA 102, as well as PHBCA107 and PHBCA126, carrying the CaPHM7-GFP allele, were grown overnight in SD-URA medium, serially diluted by 10 times and spotted onto YPD plates indicated. b Visualization of GFP signals within PHBCA107 cells of log-phase yeast-form and filamentous form induced in YPD + 10% FBS (top left), Spider (bottom left) or Lee’s (bottom right) media at 1000 × magnification on a Nikon ECLIPSE 80i microscope. Visualization of the wild type CAI4 cells (top right) as a control for hyphal morphology and green auto-fluorescence. DIC, differential interference contrast. The scale bars represent 5 μM

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