Table 1 Primer sequences of macrophage genes used for qRT-PCR assays
Gene name | Forward primer | Reverse primer |
|---|---|---|
b-actin | 5’-CGCCACCAGTTCGCCATGGA-3′ | 5’-TACAGCCCGGGGAGCATCGT-3 |
Il1b | 5’-CAACCACACAAGTGATATTCTC-3′ | 5’-GGATCCACACTCTCCAGCTGC-3 |
Il6 | 5’-TCCAGTTGCCTTCTTGGGAC-3’ | 5’-GTACTCCAGAAGACCAGAG-3’ |
Tnf | 5’-CACAGAAAGCATGATCCGCGA-3’ | 5’-CGGCAGAGAGGAGGTTGACTT-3’ |
Il10 | 5’-TGGCCCAGAAATCAAGGAGC-3’ | 5’-CAGCAGACTCAATACACACT-3’ |
Ifng | 5’-TGAACGCTACACACTGCATCT-3’ | 5’-CGACTCCTTTTCCGCTTCCTG-3’ |
Mcp1 | 5′- GGTCCCTGTCATGCTTCTGGG-3’ | 5’-TCCAGCCTACTCATTGGGATC-3’ |
P50 | 5’-CTCACTCAATATTTAATGCAG-3’ | 5’-CCCTCCGTGTGATGGGCCTTC-3’ |
P52 | 5’-CCCATGGAGGTTTGCCAGGTG-3’ | 5’-CCCACCAGACTGTGGGCATGC-3’ |
P65 | 5’-CATCCACATGAACTTGTGGGG-3’ | 5’-CTGGCTAATGGCTTGCTCCAG − 3’ |